yhdR [2018-02-06T19:40:12.000Z]

similar to aspartate aminotransferase

locus: BSU09570

pI: 5.01

mw: 43.73 kDa

protein length: 393 aa

gene length: 1179 bp

function: unknown

product: unknown

essential: no

ec: 2.6.1.1

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,034,046 → 1,035,227

The protein

Catalyzed reaction/ biological activity

• L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (according to Swiss-Prot)

Protein family

• class-I pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|AlaT] (31%)

Structure

• [PDB|3ELE] (from Eubacterium rectale, 34% identity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• The gene is annotated in KEGG as aspartate aminotransferase EC 2.6.1.1. In MetaCyc the protein is marked as “similar to aspartate aminotransferase”. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is availablavailable. [Pubmed|19935659]

Expression and Regulation

Operons

• genes: [gene|8F7432F7EF0F767C6000D553A3ACE29FBA2EDCEE|yhdR]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B485 (yhdR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1484 NBRP B. subtilis, Japan]
• BKE09570 (Δ[gene|8F7432F7EF0F767C6000D553A3ACE29FBA2EDCEE|yhdR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTCCCTTCCGCATAT, downstream forward: _UP4_TAAGAAGGCAAAGAAAAGAA
• BKK09570 (Δ[gene|8F7432F7EF0F767C6000D553A3ACE29FBA2EDCEE|yhdR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTCCCTTCCGCATAT, downstream forward: _UP4_TAAGAAGGCAAAGAAAAGAA